MLM Directory

Name the year Adam Triester announced on the Purdue Cytometry Mail List his need for help in developing FlowJo?

RE: Report from ISAC Meeting San Diego From: Adam Treister (adam@treestar.com) Date: Wed May 22 2002 - 01:45:52 EST Next message: Susan DeMaggio: "Re: Core Manager's Workshop" Previous message: Simon Watson: "Computer Networks" Maybe in reply to: J.Paul Robinson: "Report from ISAC Meeting San Diego" Next in thread: Adrian Smith: "Biotinylation reagents" Reply: Adrian Smith: "Biotinylation reagents" Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] -------------------------------------------------------------------------------- > From: J.Paul Robinson [mailto:jpr@flowcyt.cyto.purdue.edu] > Subject: Report from ISAC Meeting San Diego > Colleagues: > Hello from sunny California and the ISAC XXI congress. This message comes to you > from the CYBER CAFE generously provided by Adam Triester of Tree Star, Inc of FLOJO land! > Adam has made a bank of 12 computers, a wireless network and, lots of network cables for > laptops. He has a T1 fast line and is providing FREE access for the entire congress. > The room is ALWAYS full and is definitley the most popular place in the congress. > It has nothing to do with the outstanding FREE coffee from Ryan Bros, Coffee again > generously provided by Adam. This is the best facility provided by any vendor ever!..... > so long live FLOJO..... and more free coffee and internet access.... Paul, Thanks for the kind words, but I can't take all the credit for the CyberCafe. Apple generously provided all the Macs. IT departments around the world may say that Macs are hard to network, but we put a dozen Macs on the Internet in under an hour. Apple sent top of the line G4s with Cinema displays, and Titanium PowerBooks, as well as a bunch of iMacs. Imagine what the lines would have been like if we only had three computers, as we did in Montpelier. The true highlight of the cafe was the free espresso. Thanks to Phoenix Flow Systems, Guava Technologies, PROzyme and Becton Dickinson for contributing to the coffee fund. By the time we got to the coffee I had blown the marketing budget on this endeavor, and these companies stepped in to make sure you had Ryan Brothers coffee instead of the swill we'd have gotten from the hotel. I also extend a special thanks to Kevin Becker for bringing the Mar Dels to the banquet. The best band of any ISAC I've attended. The CyberCafe crew was Jennifer Wilshire, Maciej Simm, Adam Treat and Amy Hsu. They thought they were getting a leisurely week on the beach, and ended up working 9 to 9 every day to keep the CyberCafe running. It was an exhausting schedule, and they were tireless in their support of the attendees' Internet needs. It never would have come off without them. Sophia Ascani and Alexandra Treister tie-dyed the 350 shirts. Each one is a unique work of art, and each was hand-dipped. Our garage floor has the stains to prove it. So wear them proud, and wash them in cold water. We've agreed to do it again at ISAC XXII in Montpelier. I'm just thankful this congress is only held every second year. All this marketing crap just gets in the way of my programming. Au revoir, Adam ------------------------------------------------------------------ Adam Treister Tree Star, Inc. ph: 800-366-6045 intl: 1-650-591-2854 fax: 1-650-508-9186 adam@treestar.com www.flowjo.com ------------------------------------------------------------------ -------------------------------------------------------------------------------- Next message: Susan DeMaggio: "Re: Core Manager's Workshop" Previous message: Simon Watson: "Computer Networks" Maybe in reply to: J.Paul Robinson: "Report from ISAC Meeting San Diego" Next in thread: Adrian Smith: "Biotinylation reagents" Reply: Adrian Smith: "Biotinylation reagents" Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] -------------------------------------------------------------------------------- This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:41:43 EST a tractor and other farm tools From: Larry Seamer (la...@athena.unm.edu) Date: Fri Jul 22 1994 - 15:12:34 EST • Next message: Brian Hall: "Employment Opportunity" • Previous message: Larry Seamer: "CD-ROM" • Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] ________________________________________ The time has come the walrus said to speak of many things of shoes and ships and sealling wax of cabbages and kings of why the B-D is giving not and whether a tractor sings. I finally feel philosophical enough to impose some of my thoughts regarding the recent B-D software debate on my colleagues. I use B-D in this discussion as a paradigm for all flow manufacturers and biotech companies. Many of the arguments I've read on this network seem to reflect a disdain for business in academia. In other words, the notion exists that a profit motive is less than honorable and often interferes with legitimate scientific inquiry. I contend that corporate profit is in the best interest of academic science. B-D makes money by selling cytometers, not software. Software sales can not support the overhead necessary to build the hardware. Every company tries to get a "leg-up" on their competition with innovation, low price, more features, etc. We are the beneficiaries of this competition. I concede B-D's point, giving away their 'leg-up' will in the end be counterproductive to B-D and to those of us who rely on B-D. If B-D does not make money, they go away and we all build Cytomuts. I am not very handy, so, this institution would probably be flow-less if someone did not sell cytometers. Whether that's a good thing or a bad thing is fodder for future discussions. Many of us have been in this field long enough to remember when our monoclonal antibodies were limited, quality was inconsistent, standardization was poor and support was nonexistent. Also, new antibodies were slow in appearing. Thankfully, it is profitable to sell monoclonal antibodies and the research which requires them has flourished. One final point on software. As some have noted in past messages, the best software has come from independent providers (Verity, Phoenix, TrueFacts, TechTeam, etc). If software is given away, it will no longer be feasible to sell it and those who make a living by providing us with innovative and useful software will go the way of the Dodo. We will be left with only those who are willing to give it away or (B-D, Coulter and Ortho). As those companies have argued, they do not make money on software. Therefore, it is unreasonable to think that we can count on the flow manufacturers to meet our analysis needs. That leaves only our colleagues who are willing to give it away as a source new software. I hope the parallel to monoclonal antibodies is apparent. ________________________________________ • Next message: Brian Hall: "Employment Opportunity" • Previous message: Larry Seamer: "CD-ROM" • Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] [ attachment ] ________________________________________ This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 - 17:27:06 EST Re: Thanks for the suggestions - was rendering in 3D This message: [ Message body ] [ More options ] Related messages: [ Next message ] [ Previous message ] [ In reply to ] [ Next in thread ] [ Replies ] From: Adam Treister <...@treestar.com> Date: Thu May 18 2006 - 20:20:18 EDT On May 12, 2006, at 10:02 AM, Bushnell, Timothy wrote: > Thanks to everyone who suggested possible software to view data in > 3D. I’ll be trying several different platforms to see which works > best for our applications. > > The suggested platforms include (in alphabetical order): > > Coulters CXP software for the FC500 > > Rflowcyt > > Weasel: http://www.wehi.edu.au/cytometry/WEASELv... > > Winlist 3d from Verity House Software (www.vsh.com) > > Regards > > TimTim, With all due respect to these solutions, you shouldn't think that Mario could sleep at night if anyone could perform high dimensional analysis better than FlowJo. Disclaimer: (it shows up as a Quicktime movie), but that too is very difficult to use in a way that's better than two 2D graphs connected by a gate. If you really want to increase your dimensionality, we're just adding a new "Polyvariate Plot" to the Mac version of FlowJo for next week's ISAC. The idea was taken from RFlowCyt. We've added interface refinements to make it more interactive, but like any good R tool, it'll astound and confuse you. http://www.flowjo.com/v8/html/polyvarplo... The Polyvariate Plot can model transformations in any number of dimensions. So it will produce a 3D plot, or as many dimensions as you want (Shown below in 5D). And it projects these transformations onto a graph window, so you can gate on them. We're still trying to figure out the applications for this visualization, but if you're looking for another dimension as a way to differentiate populations, we think this is potentially much more power powerful than conventional spatial projections. This is explained in the poster P178 at ISAC next week, or at the web page above. Be forewarned: This is the opposite end of the sizzle/steak spectrum. Most people use 3D graphs to make their PowerPoints look spiffy. These graphs are absolutely impossible to explain in a presentation. Adam ----------------------- A 3D plot:  A 5D plot:  Received on Fri May 19 17:58:00 2006 This message: [ Message body ] Next message: Jerry Spangrude: "Making tandem conjugates" Previous message: Rebe...@UCHSC.edu: "Sorting dendritic cells and platelets on FACSAria" In reply to: Bushnell, Timothy: "Thanks for the suggestions - was rendering in 3D" Next in thread: Jerry Spangrude: "Making tandem conjugates" Reply: Jerry Spangrude: "Making tandem conjugates" Reply: A.J. Rossini: "Re: Thanks for the suggestions - was rendering in 3D" Contemporary messages sorte http://advenet.com/flowcytometry/blog/default.aspx

Public Comments

1. If it happened more than 12 years ago, the statue of limitations has expired.